Amphasys Pollen Analysis Instructions
Pollen Buffer & Chip Selector
Find the recommended AmphaFluid buffer, chip and filter size for measuring pollen viability of your crop. Based on Amphasys best-practice recommendations.
Reference: Amphasys Pollen Analysis Instructions — June 2026 ⇩ Download PDF version
| Species ▲▼ | Common name ▲▼ | Chip µm ▲▼ | Filter µm ▲▼ | AmphaFluid buffer ▲▼ | Alt. ▲▼ |
|---|
How to read this
- AmphaFluid buffer = recommended AmphaFluid for pollen viability measurement.
- Alt. = alternative AmphaFluid to try for other purposes (development stages, ploidy).
- ★ Crop-specific AmphaChip available for this crop — an optional dedicated chip for the Ampha P20 with automated data analysis. The standard chip works too.
- Chip & Filter size must match your pollen size. Pollen size can vary ±20% or more; polyploidy increases size.
- Templates are the AmphaSoft PRO measurement templates — contact support for the files.
Sample prep tips
- Dried / stored pollen: rehydrate and equilibrate in buffer before measuring.
- Sticky or oily pollen: add 0.05% Tween 20 or 80.
- Microspores / protoplasts: most species work with AmphaFluid 6, or dilute the isolation medium with AmphaFluid 6 (1:4 to 1:6).
- Use fresh samples where possible, filter before use, and measure immediately.
Available chip sizes
80 µm120 µm250 µm300 µm
Need a template, a species that isn’t listed, or a special application (e.g. polyploidy)? Contact support@amphasys.com.
Legend
Filter colour code (matches the physical AmphaFilter)
50 µm 100 µm 200 µm 300 µm
AmphaFluid buffer & chip
AmphaFluid 5 AmphaFluid 6 AmphaFluid 10 AmphaFluid 11 outlined = alternative buffer ★ crop-specific chip available
Buffer colours are for quick identification only and imply no ranking — every recommendation is equally valid. The filter colours match the physical AmphaFilter colour coding.
Recommendations only — best-practice suggestions; buffer ingredients may change. Source: Amphasys Pollen Analysis Instructions, June 2026. © Amphasys AG.
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