Picture collage honoring Andrea Hunn for her project in Swiss Youth in Science

Analyzing the Effects of Natural Remedies on Cancer Cell Lines

Graduate Student Honored for Conducting Research on Cancer Cell Viability on the Ampha Z32

As part of her A-level project, Andrea Hunn used an Ampha Z32 Impedance Flow Cytometer to analyze the effects of selected natural remedies on cancer cell lines. 

Her project was selected to participate in the national “Swiss Youth in Science” competition. At Amphasys we are very proud and excited about Andreas accomplishment and are looking forward to follow her progress as a young scientist. 

Analyzing the Effects of Natural Remedies on Cancer Cell Lines with Impedance Flow Cytometry​

Her research deals with the question whether the addition of natural remedies such as Wogonin, Curcumin and  Epigallocatechingallat (EGCG) can be used to observe cell death. The aim was to measure cell death with the Amphasys Impedance Flow Cytometer, which was used for such a toxicity study on human cells for the first time.

Furthermore, the influence of natural remedy concentration and incubation time on cell death was investigated. The question arose whether the natural remedies only kill blood cancer cells or also other types of cancer, but not minor degenerated human cells.

Covid_19

Support and External Maintenance in Times of COVID-19

We are all facing challenging times due to the current events around the global spread of COVID-19.

At Amphasys, we have taken all necessary measures to protect our team and are committed to providing our customers with full online support, products and services to enable their continued business operations during this extraordinary time.

Maintenance

To ensure optimal performance of the Ampha Z32, a yearly maintenance is indispensable. To respect the social distancing measures and to circumvent the current travelling restrictions we have implemented an “External Maintenance” solution. This maintenance model entails shipping of the instrument to our technical personnel, who will service it and send it back to our customers within a few days. For more information, please contact us at support@amphasys.com.

Support

Our support team is working remotely and is committed to helping you with any requests or questions you might have. Please contact us at support@amphasys.com.

Products/Consumables

And last but not least, all our products can be ordered without any restrictions at order@amphasys.com.

We hope that the global situation will soon improve.

Sorghum field

Breeding for Cold Stress Tolerance in Sorghum

Impedance Flow Cytometry Used to Analyze Sorghum Pollen Traits

A group of researchers from the Department of Plant Breeding at University of Giessen and the NPZ Innovation GmbH have conducted a study on reproductive cold stress tolerance in Sorghum F1 hybrids.

According to the study, the production of large pollen quantity with sufficient vitality also at lower temperature is considered to be the underlying physiological process to ensure seed set under cold stress in sorghum.

To quantify the amount as well as the viability of sorghum pollen, they have used the fast and non-invasive Ampha Z32 Pollen Analyzer

"Reproductive Cold Stress Tolerance in Sorghum F1 Hybrids is a Heterotic Trait"

Abstract:

The sensitivity of sorghum to pre-flowering cold stress, resulting in reduced pollen viability and poor seed set, is a major constraint for expanding growing areas into higher altitudes and latitudes. Nevertheless, compared to juvenile cold tolerance, reproductive cold tolerance in sorghum has received much less attention so far, and very little is known about its inheritance in F1-hybrids. We have composed a representative factorial (n=49 experimental F1-hybrids) for a comprehensive study on heterosis and combining ability for crucial tolerance traits as spikelet fertility (panicle harvestindex), seed yield and pollen viability, using field trials in stress- and control environments in Germany and Mexico as well as climate chamber experiments. Our results indicate a heterotic and rather dominant inheritance of reproductive cold tolerance in sorghum, with strong effects of female general combining ability (GCA) on F1-hybrid performance in our material. These findings, together with the comparatively low contribution of specific combining ability (SCA) effects and high heritability estimates, suggest that robust and efficient enhancement of reproductive cold tolerance is feasible viahybrid breeding.


(1) (PDF) Reproductive Cold Stress Tolerance in Sorghum F1 Hybrids is a Heterotic Trait. Available from: https://www.researchgate.net/publication/335627689 [accessed Feb 11 2020].

Comparison of germination assays and the Ampha Z32 for pollen viability analysis

Pollen Germination vs. Pollen Viability

Learn Why Germination Assays are Outdated

Pollen quality plays an important role in plant breeding and seed production. In-vitro germination is often regarded as the gold standard method for assessing pollen viability.

In a recent industry study on tomato pollen (Solanum lycopersicumin-vitro germination and Impedance Flow Cytometry on the Ampha Z32 Pollen Analyzer were compared.

The conclusion of this study are:

  1. Impedance Flow Cytometry (IFC) and germination show a good correlation when the correct pollen sample preparation and germination conditions are applied.
  2. IFC method is more accurate, less prone to bias and significantly faster. For the analysis of 100 samples a time savings of at least 10 hours is achieved.
  3. For optimal results, a controlled rehydration process of 30 min needs to be applied

If you are interested in the details of this comparison, please check out our Application Note.

Strong interest in pollen quality monitoring at ISF Brisbane

Strong Interest in Pollen Quality Analysis in Australia

In June the Amphasys sales team visited Australia for a demo tour with our new distribution partner ICT International. The trip started with the installation of an Ampha Z32 at the University of Sydney for use in their wheat breeding program and was followed by visits to Central Queensland University, Rijk Zwaan and University of Queensland. We were very pleased with the good discussions that we had and the overwhelming interest shown in pollen viability applications.

One of the highlights of the trip was attending the ISF World Seed Congress in Brisbane, where we had many interesting discussions on various applications – ranging from how to implement yield forecasting to how to best control the pollen quality through storage and transportation.

We thank all visitors at our booth and of course our strong distribution partners ICT International and Zealquest for their support during the meeting!