Pollen Germination vs. Pollen Viability

Learn Why Germination Assays are Outdated

Pollen quality plays an important role in plant breeding and seed production. In-vitro germination is often regarded as the gold standard method for assessing pollen viability.

In a recent industry study on tomato pollen (Solanum lycopersicumin-vitro germination and Impedance Flow Cytometry on the Ampha Z32 Pollen Analyzer were compared.

The conclusion of this study are:

  1. Impedance Flow Cytometry (IFC) and germination show a good correlation when the correct pollen sample preparation and germination conditions are applied.
  2. IFC method is more accurate, less prone to bias and significantly faster. For the analysis of 100 samples a time savings of at least 10 hours is achieved.
  3. For optimal results, a controlled rehydration process of 30 min needs to be applied

If you are interested in the details of this comparison, please check out our Application Note.

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