Single Cell Analysis in Bioprocessing
Cell analysis is an essential step in the development and optimization of bioprocessing. By understanding the behavior and the properties of the cells, the bioprocess can be optimized to improve the yield of the desired product and ensure the safety and quality of the final product.
Amphasys’ state-of-the-art technology based on Impedance Flow Cytometry offers an easy-to-use method for the analysis of single cells. Based on electrical resistance and the dielectric properties of cells in suspension, the cells under investigation do not need specific molecular markers or dyes and are not altered at all by the measurement. Implemented on microfluidic chips, this non-invasive and label-free technique can be used for fast and efficient characterization of single cells in suspension, and the cells still further processed for their destined use.
Benefits:
- No labeling, no staining, no incubation
- No calibration
- Short sample preparation, short measuring times, immediate results
- Highly sophisticated software for data analysis
- Simultaneous analysis of cell viability, counting and other cell properties
- Cell sizes between 1 µm and 50 µm
- Low operational costs
- Online measurements via bypass
For more information, please contact us.
Amphasys provides four microfluidic chips of different sizes which allow the measurement of the whole range of mammalian and (other) animal cells, bacteria, algae, yeast, and somatic cells in milk.
For other cells, please contact us.
The Ampha X30 is Amphasys’ next generation of cell analyzers for bioprocessing applications. This innovative instrument enables highly accurate determination of cell viability, concentration, cell differentiation and additional cell properties. Easy handling and the fast measurements are supported by the highly sophisticated software AmphaSoft PRO which allows to analyze measurement data in great detail and various views. The instrument is ready for online measurements, in which samples are pumped directly from the reactor through the flow cell. As the cells are not harmed by the measurement, it would also be possible, taking appropriate sterility precautions, to pump them back into the bioreactor.
