Comparison of germination assays and the Ampha Z32 for pollen viability analysis

Pollen Germination vs. Pollen Viability

Learn Why Germination Assays are Outdated

Pollen quality plays an important role in plant breeding and seed production. In-vitro germination is often regarded as the gold standard method for assessing pollen viability.

In a recent industry study on tomato pollen (Solanum lycopersicum) in-vitro germination and Impedance Flow Cytometry on the Ampha Z32 Pollen Analyzer were compared.

The conclusion of this study are:

  1. Impedance Flow Cytometry (IFC) and germination show a good correlation when the correct pollen sample preparation and germination conditions are applied.
  2. IFC method is more accurate, less prone to bias and significantly faster. For the analysis of 100 samples a time savings of at least 10 hours is achieved.
  3. For optimal results, a controlled rehydration process of 30 min needs to be applied

If you are interested in the details of this comparison, please check out our Application Note.

Download the Application Note
Amphasys at ASTA Seed Meeting 2019

Amphasys at ASTA Seed Congress 2019

On-Demand Pollination for Corn Seed Production

Discover the benefits of pollen analysis in seed production:

  • – Higher yields
  • – Increased purity
  • – Lower cost of goods

Pollen quality analysis is the key to optimizing the pollination process and hence drive yield improvements, save production costs and increase the quality of your seed. Come see our solutions on the Seed Expo Tour on Thursday at 10:00 am and at the Ag Innovative Showcase on Tuesday at 1:00 pm.

Visit the pollen experts at booths #1123 and #1125 at the ASTA Seed Congress and we are happy to show you the technologies that make the difference!

Schedule a meeting

PowerPollen harnesses optimum pollen management by providing seed producers better pollination at lower costs, resulting in a higher return on investment.

The heart of the PowerPollen solution is Amphasys’s high throughput pollen analyzer that enables data-driven pollen quality monitoring and drives yield in plant breeding and seed production.

Neutec Group offers state of the art technologies to enhance any lab performance in the Biotechnology and Agriculture laboratories. Their equipment offers unprecedented analysis for seeds, pollen, and Agro studies. At ASTA they will showcase the Ampha™ Z32 pollen analyzer, manufactured by Amphasys and utilized by Power Pollen – come see it on the Innovation Tour Thursday, December 12, 2019, 10:00 am – 11:30 am.

Marco Di Berardino will be attending the 1st ExoFlowMetry Meeting

Amphasys at 1st ExoFlowMetry Meeting in Rome

Single Cell Analysis with Impedance Flow Cytometry

The potential of flow cytometry (FC) is still large in areas different than the clinical and biomedical applications, for which it is widely applied since the ’60s of the last century. High process stability, multiparametric and analytical precision are typical features for FC, all well suited to different and unconventional (exotic) applications. New fields of application such as pollen analysis and plant breeding, diary production, brewery, winemaking, fish and animal farming, and genomics, inner and outer water quality control, are now available to FC characterization and manipulation, and more and more are fast developing. 

Amphasys as one of the leading companies for practical applications of Impedance Flow Cytometry is proud to be part of the First International Workshop on Exotic Flow Cytometry from November 13 – 15, 2019 in Rome and will present its Ampha Z32 Impedance Flow Cytometer.

Learn about our next single cell applications