Invitación para la visita virtual

Visita virtual en Español – Porque la calidad del polen ¡importa!

Porque la qualidad del polen ¡importa!

En esta sesión del Dr. Gorka Santos habla de los aspectos fundamentales del control de la calidad del polen de diferentes especies. Explica cómo el control de la calidad del polen puede crear valor, ahorrar costes y aumentar el rendimiento. Descubra por qué:

– La tecnología de Amphasys va más allá de la viabilidad del polen. Averigüe una serie de aplicaciones prácticas

– Caracterizar y optimizar la cadena de suministro de polen tiene el potencial de aumentar el rendimiento de la producción de semillas

– La validación de los protocolos de almacenamiento de polen es esencial para garantizar una calidad constante del mismo.

– El control estandarizado para el envío y el comercio de polen es fundamental.

Si desea conocer el importante papel de la calidad del polen en el cultivo de plantas y la producción de semillas, vea la grabación de nuestro seminario web

Because Pollen Quality Matters!

  • In 2020 Amphasys has organized a series of sessions on the importance of pollen quality monitoring. This ‘Virtual Amphacademy’ was a huge success and enabled us to reach an international audience of experts and researchers engaged in plant breeding and seed production.

Based on our participants’ feedback and with an increasing customer base in Latin America, we have decided to organize our first webinar in Spanish.

Use this link, to watch a recording of the English version of our webinar.

Nominated for the Green Business Award 2021

Amphasys Nominated for Green Business Award

Profitability and sustainable action do not conflict. The companies nominated for the “Green Business Award” are shaping the entrepreneurial world of tomorrow with a pioneering spirit and passion.
They are successful in the market and raise awareness for ecological solutions.

About the "Green Business Award"

The “Green Business Award” honours innovative companies that combine economic success with ecological impact. A unique selection process via national business associations and environmental organisations guarantees that the most convincing solutions are sought out each year.

With the “Green Business Award” among other formats, “Green Business Switzerland” focuses on the positive connection between entrepreneurial success and sustainable action. Behind this forward-looking cooperation of key players from business and environmental protection are, among others, the FOEN, economiesuisse, öbu, Pusch, Swissmem, the Swiss Environmental Foundation and WWF
Switzerland. [1]

Because Amphasys is Part of the Solution!

If we want to preserve our planet for future generations, business must be part of the solution!

Amphasys enables its customers in agriculture and food production to reduce energy consumption, production area and required arable land by up to 20%. More than 100 customers, e.g. BASF, Bayer, Corteva, KWS, Syngenta and many universities, already rely on our technology.

Our cytometer analyses the quality (“viability”) of individual cells. It is the only device that is portable, fast and provides all the detailed information needed, at an excellent price-performance ratio. This makes it a breakthrough technology for many applications directly in the field or in production, for which there is no other solution today.

We are honored to be nominated for this important award and eagerly await July 2, 2021 – when the finalists will be announced.

[1] Source: Green Business Switzerland. “Green Business Award”, Media Release, 20.04.2021 

A picture of beautiful apple blossoms

Apple Pollen Viability Analysis

The Important Role of Pollen in Apple Production

Apple pollen viability plays a crucial role in apple production. Viable pollen is a basic requirement for successful pollination and therefore directly influences fruit formation and quality.

The viability of pollen varies throughout the day and can be negatively impacted by many external factors such as heat, drought or the application of pesticides.  Therefore, monitoring apple pollen viability is indispensable to ensure an optimal process – from pollen collection up to artificial pollination.

Apples on tree
Picture of an apple orchard

Main Fields of Application

Monitoring pollen viability with the Ampha Z32 Impedance Flow Cytometer allows rapid and accurate pollen viability testing with minimal sample preparation. Main fields of application are:

  •  – Characterization of germplasm for pollen viability and quantity
  •  – Optimization and control of pollen harvest and storage
  •  – Control of pollen quality before supplemental pollination
  •  – Assessment of the impact of artificial pollination process on pollen viability
A picture of an apple pollen viability measurement with the Ampha Z32 Pollen Analyzer

The figure shows the result of a measurement of an apple pollen sample with the Ampha Z32. Before measuring, the pollen was stored at -20°C for 12 months. Before suspension in the measurement buffer, dehydrated stored pollen is rehydrated in a moist chamber.

Apple Pollen Application Note

Download our application note, or contact us for a free consultation offer!

Seed Central's Innovator Showcase

Amphasys at Seed Central’s Innovator Showcase

Because Pollen Quality Matters!

Seed Central’s Innovator Showcase is a joint event by Seed Central, UC Davis Innovation Institute for Food & Health, UC Davis Microbiome Special Research Program and UC Davis Venture Capitalyst.

The event offers a platform to innovative companies and startup’s working in the seed, agricultural, agbiotech, microbiome and food/health sector.

Amphasys was invited to participate in this online event with a short and concise 3 minute pitch and a more detailed ‘online company visit’.

3 Minute Pitch

Watch the recording of our 3-minute pitch and learn:

  1. why pollen quality matters
  2. how data-driven pollen quality monitoring can look like

Online Company Visit

In the recording of our online company visit we talk about:

  1. Who we are and how we analyze pollen
  2. Areas in plant breeding and seed production where our technology is used
  3. Practical application examples 
a picture of hazelnut catkins shedding pollenins

New Study on Hazelnut Pollen Phenotyping Using the Pollen Analyzer

Plant Phenotyping - The Science of Crop Characterization

Modern plant phenotyping is a rapidly emerging research area concerned with the quantitative measurement of the structural and functional properties of plants. It provides essential information to plant breeders and crop management when selecting the best genotypes that will become future cultivars well-adapted to different environments [1].

Non-destructive high-throughput methods have replaced tedious classical assays, now generating large amount of multiparametric data in a very short time. One example used for pollen phenotyping is our Ampha Z32 Pollen Analyzer, capable of analyzing thousands of pollen grains in just a few seconds.

Impedance Flow Cytometry Reveals Large Differences in Hazelnut Pollen Sterility

In a recent study, the Ampha Z32 Pollen Analyzer was used to systematically investigate two important aspects of hazelnut biology: Pollen viability and pollen sterility. Those parameters were assessed in 33 cultivars used for hazelnut production and compared to wild genotypes. The measurements allowed the researchers to reliably classify pollen grains into viable, dead and sterile in a high throughput. Interestingly, both the pollen viability and the degree of sterility were strongly cultivar-specific, and both parameters varied over a surprisingly wide range. Importantly, only a small amount of dead pollen was observed. This suggests that pollen sterility is a key determinant of pollen quality in hazelnut, and therefore deserves particular attention when selecting suitable pollinators.

Congratulations to Lorenzo Ascari and his team for this impressive work and for demonstrating the power and potential of Impedance Flow Cytometry in plant science.

If you are interested in reading the full article, please click on the button below.

Amphasys_Wheat

New Study on Embryo Yield Prediction in Microspore Cultures

Coping with Increasing Demand for Wheat

Wheat (Triticum aestivum L.) is the second most important staple food crop worldwide, and it is accounting for a considerable amount of global human calorie consumption. Due to population growth and dietary changes, the demand for wheat is predicted to increase by up to 70% until 2050 [1]. Besides optimizing plant protection and plant nutrition, plant breeding exhibits a key role in increasing the productivity per land area.

Hence, new approaches and technologies are needed to meet this increasing demand.

Embryo Yield Prediction in Microspore Cultures with Impedance Flow Cytometry

In a recent study conducted by the applied research center Vegenov, the Amphasys Impedance Flow Cytometer Ampha Z32 was successfully used to characterize the developmental pathway of the microspores during the androgenesis process as well as to predict the embryo yield during the early stages of microspore cultures in wheat. The technology has proven to be a robust and reliable tool for embryo yield prediction within the first seven days of in vitro culture.

Impedance Flow Cytometry Allows the Early Prediction of Embryo Yields in Wheat (Triticum aestivum L.) Microspore Cultures

Abstract :

“Haplomethods are key biotechnological tools that make it possible to rapidly produce perfectly homozygous lines, speeding up plant breeding programs. Under specific stress conditions, microspores are reprogrammed toward sporophytic pathways, leading to embryo formation. Various endogenous and exogenous factors affect embryo yield in androgenesis, so the improvement of androgenesis efficiency requires the development of early, reliable and robust reactivity markers. During the last decade, numerous cytological, cellular and biochemical approaches were carried out to finely characterize microspore development and fate during androgenesis.

However, the different available markers are often species-dependent, and their development and application are time-consuming and cumbersome. In this study, we show the suitable use of impedance flow cytometry (IFC) to develop new robust, reliable and strong markers of androgenesis reactivity in wheat, leading to: (i) routine monitoring of the viability of heterogeneous cell cultures; (ii) quick and simple evaluation of stress treatment efficiency; and (iii) early prediction of embryo yields from microspore suspensions. IFC can therefore provide the fine characterization of all of the microspore developmental pathways that occur in a cell suspension, for embryogenic microspores as well as pollen-like microspores. IFC technology has become a very useful tool to track and characterize wheat microspores in androgenesis, but can also be adapted to other species and other in vitro cell culture systems.” [2]

Sources:

[1] CGIAR Research Program on Wheat. 2017. https://wheat.org/wheat-in-the-world/. Accessed Oct. 20. 2020

[2] J. Canonge, M.Philippot, C. Leblanc, P. Potin, M. Bodin. Impedance flow cytometry allows the early prediction of embryo yields in wheat (Triticum aestivum L.) microspore cultures. Plant Science 300 (2020) 110586.

Clostridium difficile bacteria

Quantifying Bacterial Spore Germination by Impedance Flow Cytometry

Ampha Z32 Used as a Diagnostic Tool for the Determination of Host Susceptibility to CDI (Clostridioides difficile infection)

CDI can occur as a result of antibiotic administration and consequent impairment of the gut microbiome, providing best growth conditions to C. diffiicile. This gram-positive bacterium produces toxins that can lead to severe intestinal diseases. Even though C. difficile can be treated with specific antibiotics, its spores are resistant to that treatment and can survive in the intestine. Therefore, CDI recurrence is quite frequent (20-30%!) and due mainly to germination of the dormant spores into vegetative bacteria.

The group of Prof. Nathan Swami at the University of Virginia has impressively shown how it is possible to determine the predisposition for CDI by measuring the germination of C. difficile spores to vegetative bacteria using Amphasys’ Impedance Flow Cytometer (Ampha Z32) on ex vivo cultured samples. This method is much faster (4-5 hours) and more sensitive than conventional, microbiological techniques, which normally need 24 hours for a reliable determination of the microbiome susceptibility to C. difficile spore germination. This underlines the potential of Impedance Flow Cytometry (IFC) as a point-of-care screening tool for CDI-vulnerable patients.

Please contact us, if you would like to read the full article!

Picture collage honoring Andrea Hunn for her project in Swiss Youth in Science

Analyzing the Effects of Natural Remedies on Cancer Cell Lines

Graduate Student Honored for Conducting Research on Cancer Cell Viability on the Ampha Z32

As part of her A-level project, Andrea Hunn used an Ampha Z32 Impedance Flow Cytometer to analyze the effects of selected natural remedies on cancer cell lines. 

Her project was selected to participate in the national “Swiss Youth in Science” competition. At Amphasys we are very proud and excited about Andreas accomplishment and are looking forward to follow her progress as a young scientist. 

Analyzing the Effects of Natural Remedies on Cancer Cell Lines with Impedance Flow Cytometry​

Her research deals with the question whether the addition of natural remedies such as Wogonin, Curcumin and  Epigallocatechingallat (EGCG) can be used to observe cell death. The aim was to measure cell death with the Amphasys Impedance Flow Cytometer, which was used for such a toxicity study on human cells for the first time.

Furthermore, the influence of natural remedy concentration and incubation time on cell death was investigated. The question arose whether the natural remedies only kill blood cancer cells or also other types of cancer, but not minor degenerated human cells.

Corn pollen on corn tassel

Improved Buffer for Corn Pollen Measurement

New AF8/AF9 Buffer for Pollen Analysis on the Ampha Z32 Pollen Analyzer

The Ampha Z32 Pollen Analyzer allows for a fast, reproducible and highly accurate determination of pollen viability along the pollen supply chain.

Our Amphasys R&D experts have developped two new and improved buffer for the analysis of large pollen such as corn, pumpkin, squash, zucchini and cotton. 

The new AmphaFluid buffer AF8 and AF9 improve sample preparation as they reduce sedimentation. Furthermore, a more accurate concentration measurement is enabled thanks to the new formula of the new buffers.

More information and recommendations for the measurement of pollen or microspores can be found in our “Pollen Analysis Instructions”. 

Covid_19

Support and External Maintenance in Times of COVID-19

We are all facing challenging times due to the current events around the global spread of COVID-19.

At Amphasys, we have taken all necessary measures to protect our team and are committed to providing our customers with full online support, products and services to enable their continued business operations during this extraordinary time.

Maintenance

To ensure optimal performance of the Ampha Z32, a yearly maintenance is indispensable. To respect the social distancing measures and to circumvent the current travelling restrictions we have implemented an “External Maintenance” solution. This maintenance model entails shipping of the instrument to our technical personnel, who will service it and send it back to our customers within a few days. For more information, please contact us at support@amphasys.com.

Support

Our support team is working remotely and is committed to helping you with any requests or questions you might have. Please contact us at support@amphasys.com.

Products/Consumables

And last but not least, all our products can be ordered without any restrictions at order@amphasys.com.

We hope that the global situation will soon improve.